PASTEURELLOSE CAPRINE PLUS

Seven DNA fingerprint profiles were found among 16 serogroup B strains representing other somatic serotypes. Fifty-four serogroup B isolates (isolated from classical cases of hemorrhagic septicemia) reacted with somatic serotype 2 or 5 antiserum and had DNA fingerprint profiles which resembled that of the serotype 2 reference strain. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. This characterization study adds to the considerable phenotypic variability that has been reported within the P. gallicida was demonstrated only in the avian and ovine species. septica was demonstrated in all but the leporine species while P.m. multocida was demonstrated in all the animal species and the vaccine strains. Dulcitol and sorbitol fermentation patterns meant that the isolates (including the vaccine strains) could be identified as subspecies Pasteurella multocida multocida (74%), Pasteurella multocida septica (18%), or Pasteurella multocida gallicida (8%). The isolates differed in their ability to ferment L-arabinose, D-dulcitol, D-sorbitol, D-xylose and in the production of indole and H2S in triple sugar iron agar resulting in the identification of 8 biochemical types or biovars. All isolates also fermented D-glucose, D-mannitol, and sucrose but failed to ferment lactose. Consistent results were obtained for all isolates in the test for Gram reaction, oxidase, catalase, urease, no growth on MacConkey agar and nitrate reduction. The field isolates were obtained in Nigeria from chickens (15 isolates), quail (5 isolates), cattle (31 isolates), goats (7 isolates), sheep (8 isolates), rabbits (3 isolates) and the vaccine strains (3 isolates), which are used as prophylaxis against fowl cholera and haemorrhagic septicaemia diseases.

PASTEURELLOSE CAPRINE PLUS

Phenotypic diversity among 69 field isolates plus 3 vaccine strains previously identified as Pasteurella multocida were investigated by extended phenotypic characterization. multocida type A ToxA(+) in respiratory infection among sheep. multocida type A ToxA(+) in diseased sheep found in this study is noticeable and attribute to important role of P. The high prevalence of ToxA and TbPA among diseased sheep may imply to important role of these genes in epidemiological and virulence of P. Among 4 virulence genes detected by PCR we found a remarkable high prevalence of TbPA (69.4%) and ToxA (72.2%) genes in diseased animals. Capsular typing of isolates by PCR demonstrated two capsular types A (39), D (3) including 5 untyped with 83.3%, 6.3% and 10.6% prevalence, respectively. Forty seven isolates of Pasteurella multocida were isolated from clinically healthy and diseased sheep. To learn more about the virulence of Pasteurella multocida isolated from sheep, phenotype, capsular type and some virulence factors (Pfha1, HgbB, TbPA and ToxA) of ovine Pasteurella multocida is described in this study. The study has been the sensitive levels of Ribo-PCR in epidemiological studies of pasteurellosis, however, correlation among diversity of isolates and host origin is not fully understood. multocida and understanding their interactions with host. Molecular approaches such as Ribo-PCR are necessary for determining and characterizing the diversity of P. Cluster II was divided in four sub cluster including IIa, IIb, IIc and IId.

Two minor cluster I and II was obtained with no significant diversity among the isolates. Nine isolates including JF694004.1 and 8 isolates including JF681973.1 showed 100% and 94% similarity, respectively.

Capsular type A was dominant among the isolates and was variable than serogroup D. Twenty samples representing Pasteurella phenotypes by Entero rapid kit and 17 out of 20 isolates were identified as P.multocida. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA genes. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran.

Pasteurellose caprine